The most basic working principle of pressure steam sterilizer is to thoroughly sterilize the bacteriological substances with bacteria through the water vapor under high temperature and pressure and the release of a large amount of latent heat. When the generated steam is in full contact with the articles with bacteria, the temperature can be rapidly increased by condensing into water and releasing a large amount of latent heat, which can eventually achieve the purpose of sterilization and accelerate the sterilization effect. Some of the mixture gases in the pressure steam sterilizer are closely related to the actual temperature and force. Relevant experimental results fully prove that only when the actual temperature and pressure of the mixture gas in the pressure steam sterilizer reach an optimal value, the actual sterilization effect can reach the optimal level.